Related manuals for agilent technologies dna kit laboratory equipment agilent technologies dna 7500 manual kit 24 pages laboratory equipment agilent technologies agilent 7683b installation, operation and maintenance manual 202 pages. Description number source category sub category time stamp time zone user host run ended on port 3 number of wells acquired. Laboratory equipment agilent technologies dna 7500 manual kit 24 pages. Performance characteristics of the high sensitivity dna kit. Evaluation of dna fragment sizing and quantification by. For the separation, sizing and quantification of dsdna fragments from 25 to 1,000 bp on agilent 2100 bioanalyzer. L of dna dye concentrate blue to a dna gel matrix vial red. Together with the 2100 bioanalyzer instrument, the dna kits enable the analysis of nextgeneration sequencing libraries and high resolution multiplex pcr reactions, measuring precise size and concentration of all dna fragments and smears. The d screentape has been developed for the separation and analysis of dna fragments from 35 bp to bp. Agilent d screentape and high sensitivity d screentape. Specifically, a designed dna construct is a physical.
Agilent 2100 bioanalyzer users guide for molecular assays. The d screentape system is designed for analysing dna molecules from 35 bp. The agilent dna kits, together with the agilent 2100 bioanalyzer system. Dna quality assessment faq vermont genetics network.
Agilent dna kits contain chips and reagents designed for sizing and analysis of dna fragments. Inefficient adapter ligation will result in reduced library complexity after pcr. The electropherogram of the sample well window should resemble the one shown here. Agilent technologies 12 agilent dna assay protocol 5 after completing the initial steps in setting up the assay equipment and bioanalyzer on page 7, you can prepare the assay, load the chip, and run the assay, as described in the following procedures. Similar to therin value, din dna integrity number is a software algorithm to determine dna quality. Quantitation using agilent 2100 bioanalyzer 1 purpose 1. Genomic dna contamination additional genomic dna peak genomic dna skewing 28s peak picture from agilent troubleshooting manual. The dna 500 labchip kit for analysis of dna fragments, ranging in size from 25 to 500 base pairs, and the dna labchip kit for. Dna constructs, as well as the challenges and opportunities that lie ahead. Laboratory equipment agilent technologies agilent 7683b installation, operation and maintenance manual 202 pages. The dna kits are ideal for automated sizing and quantitation of pcr fragments, restriction digests or fragmented dna. The agilent 2100 bioanalyzer is a microfluidicsbased platform for sizing, quantification and quality control of dna and rna.
Labchip dna assays are microfluidic assays that provide fast and easy characterization of dna samples for quantification and sizing analysis. For a more complete list of applications to meet your research needs. The agilent dna kit designed for the analysis of dna fragments from 25 bp was used in our work. Warning for details of safety, see the site preparation and safety manual for the agilent 2100 bioanalyzer. Many sample typ es can be analyzed on the bioanalyzer, including total rna, labeled rna, small and micro rnas, as well as small and large dna fragments. Download free agilent dna kit quick start guide agilent dna kit quick start guide thank you for downloading agilent dna kit quick start guide. You have to replace the syringe at the chip priming station with each new dna kit page. Interpreting bioanalyzer results assessments module timeline.
So, i wanted to filtrate 1 matrix vial with another column already used but it has residual. Note that the lower fluorescence for the dna chip did not affect calculation. The dna assay is capable of analyzing amplified dna fragments in the range of 25 bp, and in the concentration range of 0. Agilent dna 7500 and dna 12000 kit 1 table 1 physical specifications type specification analysis time 30 minutes samples per chip 12 sample volume 1 l kit stability 4 months see box for storage temperatures kit size 12 sampleschip 300 sampleskit table 2 analytical specifications type agilent dna 7500 agilent dna 12000. Note for best results ensure that all reagents are allowed to equilibrate to room temperature for 30 min prior to use. Po k i yuen, taku sakazume, paolo fortina, larry j kricka, peter wilding, evaluation of dna fragment sizing and quantification by the agilent 2100 bioanalyzer, clinical chemistry, volume 46, issue 11. Dna analysis with the agilent 2200 tapestation system technical overview. The platform is the industry standard for rna sample qc and has mostly replaced laborintensive gel electrophoresis. Characteristics of good labeled crna f l u o r e s c e n c e. Adaptor ligation is not 100% efficient so the dna assay can be used toadaptor ligation is not 100% efficient so the dna assay can be used to visualize the size shift after successful ligation of adaptors. Quantitative data from agilent 2100 bioanalyzer sample c dna main peak 3000 bp pcr 61. Agilent bioanalyser high sensitivity dna 5500pgl, 507000bp 1. Dna samples can be analyzed on agilent dna, dna 7500, dna 12000 or.
This online manual provides novice and advanced users with information needed to successfully run molecular assays. Feb 15, 2016 the agilent dna kit designed for the analysis of dna fragments from 25 bp was used in our work. The agilent 2100 bioanalyzer is marked with this symbol when the user should refer to the site preparation and safety manual in order to protect the agilent 2100 bioanalyzer against damage. This kit should be used in conjunction with a labchip gx touch instrument, and is used for dna ranging from 25 basepairs in size. When analyzing a mixture of different pcr products, the dna kit outperforms. The agilent 2200 tapestation system is a complete solution for automated, fast, and reliable electrophoresis. The agilent 2100 bioanalyzer system, an automated onchip electrophoresis system, is widely used for sizing and quantification of dna fragments, during ngs library preparation.
The high sensitivity d screentape system is designed for analysing dna molecules from 35 bp. Agilent 2200 tapestation system automated dna electrophoresis with scalable throughput the agilent 2200 tapestation system provides automated, fast and reliable rna, dna and protein electrophoresis. Manufacturers specifications for the size determination accuracy and reproducibility of this product are relative standard deviation rsd of 10% and 5% for a ladder as a sample, respectively. Agilent dna kits are designed for use with the agilent 2100 bioanalyzer only. Agilent dna kit from agilent technologies biocompare. Operation and maintenance of the agilent 2100 bioanalyzer for dna. For the separation, sizing and quantification of dsdna fragments from 100 to 7,500 bp on agilent 2100 bioanalyzer. Maybe you have knowledge that, people have search hundreds times for their chosen readings like this agilent dna kit quick start guide, but end up in malicious downloads. Dna quality assessments are essential for analysis using massively parallel sequencing. Iowa state university dna facility isu dna facility. Perform automated dna electrophoresis on the 2100 bioanalyzer system. Page 4 agilent dna kit quick start guide preparing the geldye mix allow dna dye concentrate blue and dna gel matrix red to equilibrate to room temperature for 30 min. Agilent bioanalyzer 2100 the agilent bioanalyzer uses a microcapillary based electrophoretic cell that allows rapid and sensitive investigation of nucleic acid samples.
Each chip can assay 1 to 12 samples with minimal sample consumption. The agilent dna kit provides higher resolution of smaller fragments. High resolution dna analysis with the dna 500 and dna. Read book agilent dna kit guide agilent dna agilent technologies setting up the assay equipment and bioanalyzer before beginning the chip preparation protocol, ensure that the chip priming station and the bioanalyzer are set up and ready to use. Agilent dna 21 checking your agilent dna assay results 6 dna ladder well results major features of a successful ladder run are. Visualized din and associated dna quantity is shown below picture adopted from agilent application. They allow separation of dna fragments up to 12000 base pairs. Our goal is to provide investigators in our jupiter, florida campus as well as well as external investigators the latest technologies in next generation sequencing ngs. Agilent has developed several dna kits 12000, 7500, and, as well as the high sensitivity dna kit, to perform sizing of dna. In addition to the dna 500 and dna kits, two other dna labchip kits are available. Good measurement practices for dna analysis with the. Agilent high sensitivity dna kit quick start guide equipment supplied with the agilent 2100 bioanalyzer system chip priming station reorderno 50654401 ika vortex mixer additional material required not supplied pipettes 10. Four rna or dna samples were run in triplicate on rna 6000 nano, dna high sensitivity hs, and dna chips according to the rna 6000 nano or dna hs kit protocols, respectively. Welcome to the genomics core at scripps research, florida.
As little as 1ul at 200 pgul is required per analysis. Perform highly accurate and precise dna electrophoresis with the bioanalyzer dna analysis solutions. The characterization of four gene expression analysis in. At this time the bioanalyzer 2100 in the core facility is not equipped with a flow cell needed for cell fluorescence analysis. Optimization of multiplex pcr on a 19plex pcr page 29 2008 data kindly provided by qiagen gmbh, germany. After loading the sample on the agilent chip of choice, the sample moves through microchannels and sample components are electrophoretically separated.
The agilent 2100 bioanalyzer is a microfluidicsbased platform for sizing, quantitation and purity assessment of dna and rna samples. In addition, we discuss emerging applications enabled by largescale. I purchased an agilent dna kit to use in the bioanalyzer, but i messed up with one spin filter. Compare agilent dna kit from agilent technologies on. Agilent dna agilent technologies agilent dna assay protocol after completing the initial steps in setting up the assay equipment and bioanalyzer on page 6, you can prepare the assay, load the chip, and run the assay, as described in the following procedures. Research use only not for use in diagnostic procedures. Dna kit further extends the dna prod uct portfolio for sizing, quantification and quality control of dna with the. Agilent dna kits contain chips and reagents designed for sizing.
L with compatible tips filterfree, nonautoclaved tips. Many sample typ es can be analyzed on the bioanalyzer, including total rna, labeled rna, small and. Agilent dna 7500 and dna 12000 kit quick start guide. The 2200 tapestation system is straightforward to use.
Agilent has developed several dna kits 12000, 7500, and , as well as the high sensitivity dna kit, to perform sizing of dna. A overlaid electropherogram traces of all rna and dna agilent bioanalyzer runs. The agilent 2100 bioanalyzer is a microfluidsbased platform replacing standard gel electrophoresis for rna sample qc, dna fragment analysis, and sdspage electrophoresis for proteins. Allow dna reagents need to equilibrate to room temperature for 30 minutes before use. The following is a partial list of applications for agilents labonachip technology for rna, dna, and protein analysis. Four rna or dna samples were run in triplicate on rna. Dna electrophoresis, dna fragments, bioanalyzer agilent. Dna size c application note marine biological laboratory. Agilent 2100 bioanalyzer university of oklahoma health.
Dna 12chip 300 sampleskit dna kit 35 minutes 12 dna 7500 kit 30 minutes 12 4 months see box for storage temp. Allow high sensitivity dna reagents need to equilibrate to room temperature for 30 minutes before use. Agilent high sensitivity d screentape system quick guide. Download agilent technologies dna kit quick start manual. Contents 3 index welcome welcome to the agilent 2100 bioanalyzer users guide for molecular assays. Agilent screentape devices ensure straightforward operation of the tapestation systems in your ngs workflow, giving you reliable results within 1 to 2 minutes per sample. Dna labchip kit sizing and quantitation 25bp small pcr products, small restriction digests protein 200 plus labchip kit sizing and analysis 14200 kd proteins dna 500 labchip kit sizing and quantitation 25500bp pcr products, restriction digests the agilent 2100 bioanalyzer cell fluorescence labchip kit. Agilent technologies dna kit quick start manual pdf. Evaluation of dna fragment sizing and quantification by the agilent 2100 bioanalyzer. Please ask a member of genomics staff to change the electrodes, then wash the electrodes. Take electrode cleaner labelled rnasezap and fill one of the wells with 350 l of rnasezap. Good measurement practices for dna analysis with the agilent. The agilent bioanalyzer 2100 system allows for accurate quantitative and qualitative assessment using small sample volume inputs. The agilent tapestation systems enable easy, economical, and efficient quality control of your dna or rna samples.
203 1234 29 523 51 1482 248 1360 1428 294 207 951 1392 917 821 905 252 1373 1035 769 246 958 1600 934 1009 1461 186 1438 337 1376 610